Illustration from Scientific Research

Experimental design. U2OS cells co-express the target peptide fused to GFP and a fusion between the specific binder fused to mScarlet and a mitochondria-targeting sequence (Mito-Tag). If binding occurs in cells, the GFP signal is relocalized to the mitochondria, whereas control cells that do not express the binder show a cytosolic GFP signal.

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