Workflow from Scientific Research

The input for CenFind is a multi-channel z-stack, which is max-projected along the z-axis. Channels of interest are selected and passed onto the respective models for centriole/procentriole/PCM detection with SpotNet (left) and nucleus segmentation with StarDist (right), before saving as coordinates (left) or masks (right). For cell scoring, the nuclei are assigned to centrioles/procentrioles/PCM using a distance metric; the analysis can be carried out using the original image data, together with the saved positions of centrioles/procentriole/PCM.

DOI