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The number of COs, marked by MLH1 foci (red), was significantly reduced in cKO diplotene-like oocytes compared with Ctrl oocytes. Twenty-five Ctrl oocytes and thirty-three cKO oocytes were obtained from 3 animals. Significance was determined by unpaired Student’s t test; **** P < 0.0001. The error bar represents the mean ± SEM.
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