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Fractions of TAD boundaries overlapping with synteny breaks identified in comparisons of G. kirkii vs. G. arboreum and G. kirkii vs. G. raimondii are statistically higher than those in multiple randomization controls, which involve groups of shuffled TAD boundaries (identified synteny breaks are maintained), shuffled synteny breaks (identified TAD boundaries are maintained), and both TAD boundaries and synteny breaks shuffled simultaneously. The maximum p value of Fisher’s two-tailed test in comparison to the respective control is still less than 0.001.
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