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The transcript level of desB determined by RT-qPCR in the wild-type strain EC1, dzrR mutant, and dzrR mutant with expression of dzrR or various dzrR homologs. Fold change of desB expression was analyzed using 2 −∆∆CT method, with the 16S rRNA gene serving as the endogenous control and EC1(pBB) as the reference sample. Data are presented as mean ± SE, n = 4. Statistical analysis was performed using permutation test versus ∆ dzrR (pBB). * P < 0.05.

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