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Experimental design for microbiome analysis of horizonal and vertical transmission of Rickettsia raoultii. In the horizontal transmission, two R. raoultii-positive cohorts and one negative cohort of Dermacentor marginatus nymphs fed on SPF BALb/c mice, including 8 mice for the first positive colony (colony 1), 8 for another positive colony (colony 2) and 8 for the negative colony (colony 3). Each Balb/C mice was fed by around 15-20 nymphs from the same cohort. In the vertical transmission, R. raoultii positive (n = 6) and negative (n = 6) D. silvarum cohorts were used. The 12-month timescales indicate the experiments carried out during the 3 years

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