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Quantitative analysis of SFB abundance in tissue biopsies using two primer sets (published by Jonsson [29] and by Snel [54] and the newly designed primer set; unpublished data, Pamela Schnupf). Quantification of SFB in human tissue biopsies was performed using the MasterMix SensiFAST SYBR (BIOLINE) and 3 different primer sets: for_5‘-TGTGGGTTGTGAATAGCAAT-3‘, rev_5‘-GCGAGCTTCCCTCATTACAAGG-3’, for_5‘-AGGAGGAGTCTGCGGCACATTAGC-3‘ ,rev_5‘ TCCCCACTGCTGCCTCCCGTAG-3‘ and for,_ 5’-TGTAGGTTGTGAAGAACAAT-3’, rev_ 5’-GCGAGCTTCCCTCATTACAAGG-3’ ( modified primer sequences, unpublished data, Pamela Schnupf).
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