The read counts of H3K27me3 in ChIP-seq (Chr20:52,317,726–52,320,951) was shown in upper panel. The single-molecular resolution of Chr20:52,317,726–52,320,951visualize each molecular methylation statues of H3K27me3. The rows in heatmap represented the different DNA molecules. The color indicated the methylation ratio (non-sliding 50-bp bins), which represented the possible H3K27me3 signal. The DNA molecules could be classified into three states: heavy state (methyl) (We used the m. 6 A methylation probability cut-off 0.80 in the single-molecular analysis; the methylated bins were defined as the methylation ratio > 0.5 in bins and at least 2 methylation sites; heavy states were defined as the methylation bin density > 75%), medium state (defined as the methylation bin density 25 ~ 75%), and light state (defined as the methylation bin density < 25%). The right heatmap showed the CpG methylation distribution on the corresponding DNA molecules in the left H3K27me3 heatmap (one-to-one matching). The H3K27me3 signal decreased with the decreasing CpG methylation (scatter plot).
