Bar Plot from Scientific Research

We simulated the reads with different length based on the pericentromeric regions (PE50-paired end sequencing 50 bp, SE50-single end sequencing 50 bp) (Methods). The pericentromeric region is defined as the boundary regions of the centromeres which could be labeled by H3K27me3 (The center areas of centromeres cannot be labeled; reference genome: CHM13). The multiple alignment rates decreased with the increasing read length. The PE150 still have 25% multiple alignments in the pericentromeric regions.

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