Gel from Scientific Research

Methylation of linear lambda DNA by pA-M.EcoGII activates m6A-site-dependent DpnI restriction endonuclease digestion. The PCR amplified unmethylated lambda DNA was treated with commercial M.EcoGII, no enzyme, and pA-M.EcoGII. The G A TC m6A methylation-dependent restriction endonuclease DpnI digestion suggested the comparable methyltransferase activity of the commercial M.EcoGII and our recombinant proteins. DNA marker: 100 bp ladder, 100–1510 bp(left); 1 kb ladder, 250–10,000 bp(right).

DOI