Electrochemical evaluation of Cyt.c concentrations in different compartments of a single Hela cell. A) Top: (T)-bright field image of the cell and Calcein-AM stained single cell that included marked red circles as sites positioning the Cyt.c sensing nanoelectrode: (I) extracellular position (II)(IV), different mitochondrial positions, (V) nucleus site and (VI) cytoplasm. Scale bar=10 m. Bottom: Voltammetric signals expressed as (I-I0)/I0) corresponding to the electrochemical analysis of Cyt.c in the respective single-cell compartments (voltammetric responses derive from the respective voltammetric waves presented in (B)). B) Voltammetric waves corresponding to the responses of the electrochemical nanoelectrode probe at the target analysis sites (marked with red circles), curve (a) in black without UV-uncaging of the probe non-active electrode. b) in red with UV-uncaging sensing-active electrode: (I) extracellular domain, (II)(IV) three mitochondrial domains, (V) nucleus and (VI) cytoplasm. Errors derived from N=4 experiments.
