Left, schematic of hippocampal slice patch-clamp recordings in CA1 pyramidal neurons in which tau was added to micropipette prior to breakthrough and allowed to diffuse intracellularly. Right, immunofluorescence images showing biocytin (green) and Tau-13 (red) in a CA1 pyramidal neuron (DAPI nuclear stain shown in blue), following in vitro hippocampal slice patch-clamp recordings, and exemplifying diffusion of soluble tau (hyperphosphorylated recombinant SF9 derived) from patch pipette into somatodendritic compartments. Scale bar represents 25 m.
