Left, no significant interaction between current step (-50 to 400 pA) and HMW tau condition (control, 0.1-10 nM) on incidence of single spikes. Two-way mixed ANOVA, CurrentStep concentration condition, F(12,172) = 0.96, p= 0.42 with lower bound adjustment. Right, significant interaction between current step and HMW tau concentration condition on incidence of burst events. Two-way mixed ANOVA, CurrentStep concentration condition, F(12,172) = 6.62, p= 0.001 with lower bound adjustment. Note markedly reduced incidence of bursts in 10-nM HMW tau condition. Data denotes average over series/time during first 50 ms of current step, with error bars as SEM, from N= 11 (control), 11 (0.1 nM), 14 (1 nM), and 11 (10 nM) cells. The control condition pertains to HT7 immunodepleted fraction prepared using the same volume as for the 0.1-nM HMW tau condition solution.
