Line Plot from Scientific Research

Association and dissociation phase curves of the binding of increasing concentrations of Q44c-HL488 (0–200 nM) added at time zero to HEK293T membranes overexpressing NanoLuc-EGFR. At 30 min, 100 nM EGF was added to each well and the dissociation phase then followed. For (B)–(D), data are shown as mean ± SD of the pooled data from five separate experiments performed in triplicate. For (E), data show mean ± SD of triplicate determinations in a single kinetic experiment. Similar data were obtained in four further repeat experiments.

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