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Illustration of the DDM setup used for live feedback imaging of migratory subpopulations. In DIA, cells were imaged every 10 min and analyzed post-acquisition. Cells were analyzed (total N = 24,940) in multiple migration properties and categorized into slow and fast (<10thand >90thquantiles, respectively) in terms of mean speed. In DDA, the subsampling of the two categories for high-magnification imaging was aided by an automated water dispenser.

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