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In vitro processes of rolling-circle replication (RCR) and stcLFR. DNA from bacteria and fungi was extracted and amplified using designed rDNA primers. Amplicons were turned into long concatemers with circularization and RCR. The products of RCR were labeled with unique barcodes by integrating transposons and hybridizing onto 30 million different clonal barcoded beads in a single tube. After PCR, these sheared short sub-fragments were subjected to DNA sequencing.

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