Forster resonance energy transfer by fluorescence lifetime imaging (FRET-FLIM) analysis reveals direct interaction between ATSK32-GFP and OPS-mCherry in the cytoplasm and at the PM. All individual data points are plotted. Horizontal bars represent the means, and error bars represent SD.n, number of root epidermal cells analyzed. A two-tailed Student’s unpaired t test analysis was used to determine the significant differences.
