Quantification of calcium transients' amplitude elicited by pneumolysin pressure application. Paired line plots showing responses of individual cells to absence of stimulation (corresponding to spontaneous activity, on the left in black circle) or 3 successive 1-s stimuli of aCSF (gray circle) versus 0.05 mg/mL pneumolysin (Ply, blue circle). Two-factor ANOVA, treatment factor F = 66.185, p = 1.47 x 10-12, Tukey HSD post hoc testing,* * *p < 0.001. Red bar: median deltaF/F (median spontaneous activity = 0.03%; median Ply = 17.49% and median aCSF = 0.19% for stimulation 1; median Ply = 18.78% and median aCSF = 0.03% for stimulation 2; median Ply = 17.20% and median aCSF = -0.20% for stimulation 3), 16 responding cells out of 17.
