Bar Plot from Scientific Research

In vitro validation of putative hits from the primary screen using ELISA and immunoblot (IB) in 293T cells. Quantification of the average tau protein level measured by ELISA. Each bar above a gene represents an individual shRNA. The effect of candidate-targeting shRNAs was normalized to the effect of non-target (NT) shRNAs. Genes were selected as tau modifiers if two independent shRNAs (p < 0.1) reduced or increased tau levels by >15% (indicated by blue and red dotted lines). Genes with white bars represent candidates with non-significant effect.

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