Gel from Scientific Research

Left: SDS-PAGE analysis of size-exclusion chromatography (SEC) fractions for the separation of Ub-Cy3Ub-Ubnubiquitin chains, containing mixtures of various chain lengths and visualized by Cy3 fluorescence. Right: dependence of the initiator insertion into the Cdc48-UN pore on the length of the K48-linked polyubiquitin chain. Bars represent the percent decrease in Cy3-donor fluorescence when UN is present in a sample consisting of one Ub-Cy3Ub-UbnSEC fraction A-G (shown in the gel at the right) andCy5Cdc48 in ATPS. Individual fluorescence values and their mean are shown for N = 3 technical replicates.

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