Line Plot from Scientific Research

Fluorescence emission spectra after 480-nm excitation ofCy3Ub-Ub3polyubiquitin chains incubated with Cdc48 andCy5UN together or individually in ATPγS. Fluorescence intensity for each spectrum was normalized relative to the Cy3 emission maximum at 568 nm forCy3Ub-Ub3alone, which was set to 1 (blue). Arrows indicate the direction of changes in Cy3- or Cy5-fluorescence intensity during the transition from a low-FRET to a high-FRET state upon binding of the most proximal ubiquitin to the UT3 domain of Ufd1.

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