Line Plot from Scientific Research

Fluorescence emission spectra after 480-nm excitation of Ub-Cy3Ub-Ubnpolyubiquitin chains incubated withCy5Cdc48 and UN together or individually in ATPγS. Fluorescence intensity for each spectrum was normalized relative to the Cy3 emission maximum at 568 nm for the Ub-Cy3Ub-Ubnalone, which was set to 1 (gray). Arrows indicate the direction of changes in Cy3- or Cy5-fluorescence intensity during the transition from a low-FRET to high-FRET state upon pore insertion of the initiator ubiquitin.

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