Bar Plot from Scientific Research

Mutations in Npl4’s initiator-binding groove compromise the Cdc48-UN-mediated unfolding of an initiator ubiquitin within K48-linked chains. Bars represent the percent increase in steady-state Cy3-donor fluorescence when wild-type or mutant UN is present in a sample ofCy3Ub-Cy5Ubnand Cdc48 in ATPγS. Individual measurements, their mean, and standard deviation are shown for N = 3 technical replicates. Statistical significance was calculated using a one-way ANOVA test:****p < 0.0001.

DOI