Gel from Scientific Research

35S-methionine labeled TAAR5-SA+70 RNCs were mixed with GTP, the slowly hydrolyzed GTP analog GMPCPP or nothing (Ø), then incubated with SPCs containing EMC3(216C)-FLAG or lacking EMC (Δ). Pelleted SPCs were subject to BMH crosslinking as indicated and analyzed directly. The non-glycosylated (-glyc) and glycosylated (+glyc) products and crosslinks to Sec61β, EMC3 (upward green arrows), SRP54, and a lumenal protein are indicated.

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