Illustration from Scientific Research

Top: diagram of HA-ASGR1 containing or lacking a zinc finger from ADR1 (ZNF) immediately after the SA (ZNF-74) or after 150aa (ZNF-224). The folded ZNF structure (PDB:2ADR39) is shown with key residues required for Zn2+-dependent folding in teal. Diagram showing how timing of targeting influences ZNF folding and HA-ASGR1 translocation. Bottom: the HA-ASGR1 constructs from the diagram were translated in the absence or presence of SPCs and Zn2+where indicated. After translation, the SPCs were recovered by centrifugation and analyzed by SDS-PAGE and autoradiography. An aliquot of the sample lacking SPCs was analyzed directly. The glycosylated (+glyc) and non-glycosylated (glyc) products are indicated.

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