Microscopy from Scientific Research

Top: construct design and a diagram depicting the situation where the substrate emerges from a cytosolic versus Sec61-associated ribosome. Bottom:35S-methionine labeled TAAR5(TMD1-3) or 23L-P2A-TAAR5(TMD1-3) were translated in the absence or presence of canine-pancreas-derived rough microsomes (cRMs). Sec61 inhibitor ApraA was included where indicated. The TAAR5(TMD1-3) protein was recovered by IP via the N-terminal HA tag. The 23L-P2A fragment was visualized by direct analysis of total translation reactions. The +glyc and glyc products are indicated. Asterisk indicates a product seen in samples lacking SPCs and may represent ubiquitin-modified substrate. See alsoFigure S2.

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