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35S-methionine labeled TAAR5-SA and ASGR1 were translated in rabbit reticulocyte lysate (RRL) in the absence or presence of SPCs derived from wild type (WT) or EMC (E) HEK293 cells. The Sec61 lateral gate inhibitor Apratoxin A (ApraA) was included where indicated. After translation, the SPCs were recovered by centrifugation and analyzed by SDS-PAGE and autoradiography. An aliquot of the sample lacking SPCs was analyzed directly. The glycosylated (+glyc) and non-glycosylated (glyc) products are indicated. Asterisk indicates a product seen in samples lacking SPCs and may represent ubiquitin-modified substrate.

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