Line Plot from Scientific Research

Example of a genomic locus where loss of CA at theGli2/Inhbbenhancer with negative 5mC-CA association is correlated with a decrease in gene expression at the connected genes upon 5mC increase. Top panel: genome browser track (representation as inFigure 1A) including PRO-seq signal and RNA-seq signal in WT and TET TKO mESCs around an intergenic enhancer with negative 5mC-CA association. The enhancer was linked to theGli2andInhbbgenes by GREAT (pink dotted lines). The log2fold-change and adjusted p value of the expression changes, as measured by RNA-seq, are displayed. Left bottom panel: CA changes at theGli2/Inhbbenhancer in WT versus TET TKO. Same representation as (F). Right bottom panel: enhancer activity changes as measured by PRO-seq at a 5-kb window around theGli2/Inhbbenhancer in WT versus TET TKO. See alsoFigure S4.

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