Microscopy from Scientific Research
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PLA in HeLa cells transfected with V5-tagged PVT1-MP and FLAG-tagged SRSF2. Red spots indicate PVT1-MP-V5 and SRSF2-FLAG interactions (additional images inFigure S3C). Cell nuclei were stained with DAPI. Controls: anti-FLAG single primary antibody only; anti-V5 single primary antibody only; both primary antibodies were omitted. As an additional control, the PLA was performed in untransfected HeLa cells (Figure S3C). Scale bar represents 20 m.
#Microscopy#PLA#HeLa cells#V5-tagged PVT1-MP#FLAG-tagged SRSF2#PVT1-MP-V5#SRSF2-FLAG#Cell nuclei#DAPI#Anti-FLAG antibody#Anti-V5 antibody
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