Violin Plot from Scientific Research

Quantification of the in vivo assay demonstrated in (E). Each data point is an independent replicate. All aromatic residues exhibited in (A)-(D) (F283, F287, F394, H566, Y462, Y567, and F605) were mutated to alanine in Cas8c aromatic mutant. All positive residues exhibited in (A)-(D) (K607, K608, R408, R205, K459, R205, R58, K56, and K55) were mutated to alanine in the Cas8c positive mutant. The interference efficiency of mutants was significantly decreased compared with WT. Mutants are statistically significant to WT (*p = 0.006,***p = 0.0008).

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