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Cerebellar tissues from 5-week-old WT and Kctd7 –/– mice were lysed in NP-40 lysis buffer and calpain 1 and CAPNS1 were immunoprecipitated and probed for interaction with α-spectrin by immunoblotting ( f–h ). Lysates from same tissue samples were also immunoblotted for α-spectrin ( f–i ) and p35 ( j–l ) to evaluate calpain-mediated cleavage, n = 4. Data represent means ± SEM; * P < 0.05, ** P < 0.01, ns not significant.

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