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Schematic illustration of the strategies for the collection of single cells, transcriptome analyses and experimental validations used in this study. Only extraembryonic tissues and placentae within the area circled by dashed lines were digested to single cells. Left, representative images of sampled mouse EPC/placentae collected at the indicated time points. Scale bars: 500 m (E7.5, E8.5); 1000 m (E9.5, E10.5, E11.5); 2000 m (E12.5, E13.5, E14.5). EPC ectoplacental cone, P-TGC parietal trophoblast giant cell, Pl placenta, Em embryo.

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