Gel from Scientific Research

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Increased and decreased beta-catenin palmitoylation by PA and 2-BP, respectively. HEK293T cells transfected with Flag-beta-catenin were treated with 2-BP (100 microM) for 6 h or PA (100 microM) for 24 h and cell lysates were subjected to immunoprecipitation. Palmitoylated beta-catenin was detected with HRP-conjugated streptavidin antibody.
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