Heatmap from Scientific Research

Predicted aligned error of AlphaFold2 (left) and AlphaLink (right) on T1064 with N eff = 10 (top) and predicted structures (bottom). Light regions signify high uncertainty. Sparse restraints decrease uncertainty across the whole protein. Satisfied crosslinks <10 A CaCa highlighted in blue, borderline crosslinks (10-15 A CaCa) in yellow, and violated crosslinks >15 A CaCa in red. Possible crosslinking sites (leucines) are shown as spheres. Regions with violated crosslinks in the AlphaFold2 prediction (left) increase in certainty (darker regions). TM score improves from 0.28 to 0.86.

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