Bar Plot from Scientific Research

PSF uniformity over the FOV. PSF measurements were carried out on-axis and at a scan angle of 1.75°, which was simulated to be the maximum theoretical diffraction-limited scan angle for the total system (Extended Data Fig. 1 ). As the magnification of the objective is proportional to n, the FOV diameter decreases with increasing index. Diffraction-limited theoretical values are indicated by the red dashed lines. The PSF FWHM (±s.e.m.) was measured for eight beads for each FOV location and immersion medium. a.u., arbitrary units.

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