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The structural flexibility of MalE estimated after filtering using the distance uncertainty threshold shown in e (Supplementary Note 12 ). Error bars represent the 1 σ percentiles averaged over all dye pairs ( n = 1, MalE-1; n = 7, MalE-2 and MalE-3; n = 4, MalE-4 and n = 5, MalE-5). The residual distance fluctuations obtained from control measurements on dsDNA in one laboratory (ds dsDNA = 0.0026 ± 0.0044) are shown as a black line (gray areas represent confidence intervals of 1 σ , 2 σ and 3 σ ).
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