Box Plot from Scientific Research

The apparent ds of the peak of the population was determined graphically from BVA (eight laboratories for MalE and seven laboratories for U2AF2, respectively) ( e ) and E –τ (five laboratories) ( f ) plots ( Methods ). For U2AF2 in the holo state, the ds was assessed only for the low-FRET RNA-bound population. Boxes indicate the median and 25/75% quartiles of the data. Whiskers extend to the lowest or highest data point within 1.5 times the interquartile range. The gray area indicates the ds obtained for the dsDNA used in a previous study 18 based on measurements performed in laboratory 1 for BVA (ds DNA = 0.0033 ± 0.0033) and laboratory 2 for the E –τ plot (ds DNA = 0.0026 ± 0.0044). The horizontal red lines indicate the expected ds for a potential conformational exchange between the apo and holo states. We computed the expected change in FRET efficiency using their structural models in the PDB (Supplementary Note 6 and Supplementary Table 9 ).

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