Protein Structure from Scientific Research

Schematic of the dynamics of U2AF2. The apo state (in gray, top) undergoes fast exchange between an ensemble of detached structures of which five representative structures are displayed. A slower exchange occurs between the dynamic detached ensemble and a compact conformation (PDB ID 2YHO ) shown below. The holo state (in green, PDB ID 2YH1 ) bound to a U9 RNA ligand (in dark gray) assumes a well-defined, open conformation. Positions of cysteine mutations introduced for labeling (L187 in RRM1 and G326 in RRM2) are depicted as black spheres with the mean dye position determined by AV calculations indicated by red spheres.

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