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Open access visualization of Illustration, LSBM, Objectives, Sample chamber, Spectrometer
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LSBM set-up: the light is delivered and collected by two objectives mounted in an inverted 90 configuration. The sample is placed in a chamber (attached to a three-axis translational stage for scanning) that is separated from the surrounding immersion liquid by a thin fluorinated ethylene propylene (FEP) foil (inset). The light collected from the sample is redirected to the spectrometer (dashed box), which consists of a combination of spherical and cylindrical lenses to generate the correct beam profile as input for the virtually imaged phased array (VIPA) that disperses the light on the camera. A pure 87 Rb cell rejects the elastically scattered (Rayleigh) light.

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