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RT-qPCR for methyltransferases of m1A (TRMT6) and m5C (DNMT2 and NSUM2) on testis from control and MTX treated males, gene expression was normalized using Rpl7 and Ef1 as housekeeping genes. Statistical analysis was performed by using the two-tailed unpaired t -student’ test. Each dot represents a biologically independent sample composed by RNA isolated from individual male testis. Valures are means ± SEM.
#Bar Plot#Scatter Plot#Error Bars#RT-qPCR#Methyltransferases#m1A#TRMT6#m5C#DNMT2#NSUM2#Testis#Control#MTX#Gene Expression
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