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Illustration of the experimental design of ex vivo studies. Primary GFP+Yap+ PDAC lines isolated from KPF and KPYYF mice were infected with Ad-Cre or Ad-GFP. Equal numbers of GFP+Yap+ and Tm+Yap− PDAC cells were mixed for sequential passaging and FACS analysis every 3–4 days. Created with BioRender.com.
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