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Genetic strategy to first activate Kras G12D and delete Trp53 in the pancreas via Pdx1-Flp , and subsequently delete Yap via TAM-induced Cre-loxP recombination systems. Note that FSF-Kras G12D /Trp53 FRT and Yap flox/flox are under the separate controls of Flp and CreER, respectively. The R26-Dual reporter marks Flp-expressing KrasG12D cells with EGFP. Upon TAM-mediated CreER activation, the EGFP locus is removed, while tdTomato (Tm) is switched on.

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