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Schematic mechanism of Fc N -glycan processing by EndoS2 as determined from lineshape analysis. The affinity of EndoS2 (here E) for Fc is two orders of magnitude higher as compared to the reaction product CT n−1 , promoting substrate depletion. On-rates are responsible for increased affinity towards Fc as compared to CT n−1 , suggesting differences in the initial enzyme-ligand encounter event. Values correspond to ( f ) and Table 1 . All experiments were acquired at 600 MHz and 298 K.
#Workflow#Flowchart#Illustration#Fc N-glycan Processing#EndoS2#Lineshape Analysis#Enzyme-Ligand Encounter
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