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The quantification of NuMA signal intensity at metaphase spindle poles in B16-F1 cells normalized to the average value of the controls. The mean values with SD from three independent experiments are shown ( n = 118 and 138 for control and Dynlrb1 RNAi, respectively). Only cells that formed bipolar spindles were quantified. Two-tailed t -tests were used in ( b – d ), ( e ), ( g ), and ( j ). One-way ANOVA with Dunnett’s multiple comparisons test was used in ( h ). ns not significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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