Microscopy from Scientific Research

Immunostaining of spermatocytes prepared by the squash technique. The BubR1 signal intensities at metaphase I kinetochores were quantified and normalized by the average value of the WT controls. The mean values with SD from three independent experiments are shown ( n = 580 collected from 30 metaphase I cells for both WT and Dynlrb2 −⁄− ). Scale bar: 5 μm.

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