Microscopy from Scientific Research

Immunostaining of metaphase I spermatocytes prepared by the squash technique. The. mean values with SD of the quantification of astral MT intensity normalized by the polar MT intensity from three independent stainings for each genotype are shown. The mean values with SD from three independent stainings for each genotype are shown ( n = 64 spindle poles from 32 cells for both WT and Dynlrb2 −⁄− ). Only cells that formed bipolar spindles were quantified. Scale bar: 5 μm (1 μm, magnified panel). All analyses used two-tailed t -tests. * p < 0.05, *** p < 0.001, **** p < 0.0001.

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