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The levels of Am and m 2 G in liver individual endo-tRNA Ala, tRNA Val, tRNA Glu, tRNA Leu, and tRNA Gly (n = 3, Additional files 10 and 11). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; NB, northern blot. All results are shown as mean ± SEM. Statistical analysis was conducted using Student’s t -test unpaired

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