Bar Plot from Scientific Research

Pathogen load in tomato leaves infected with gene silenced (dsRNA-treated) and buffer-treated (control) R. solani mycelia, at 3 dpi. The Rs_GT34 -silenced and buffer-treated R. solani were used as a negative control. The pathogen load (2 −ΔCt ) was estimated as an abundance of 18S rRNA of R. solani , upon normalization with rice 18S or tomato actin gene. The graph shows the mean values ± standard error of three biological replicates. “**”indicates significant difference at p ≤ 0.01, and “*” indicates significant difference at p ≤ 0.05.

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