Gaq protein activation was evaluated by measuring BRET signal in HEK293T cells co-expressing Gaq-RLuc8, GFP2-Gγ2, and Gβ1 in the absence (0 μg) or in the presence of increasing amounts of vectors encoding P2Y 1 -R (ranging from 0.001 to 4 μg/dish), after stimulation or not with MRS2179 (10 μM) for 1 min. Results are expressed as the difference in the BRET signal measured in the presence and in the absence of MRS2179. Data represent the mean ± s.e.m. of seven independent experiments and statistical significance of MRS2179-induced BRET modulation between cells expressing P2Y 1 -R or not (0 μg) was assessed using one-way ANOVA followed by Holm-Sidak’s post-tests. (** p < 0.01; **** p < 0.0001; ns, not statistically significant).
