Clustered from Scientific Research

Basal Gaq protein activation was evaluated by measuring basal BRET signal in HEK293T cells co-expressing Gaq-RLuc8, GFP2-Gγ2, and Gβ1 in the absence (0 μg) or in the presence of increasing amounts of vectors (ranging from 0.001 to 4 μg/dish) encoding AT1-R or P2Y 1 -R. Data represent the mean ± s.e.m. of five independent experiments and statistical significance between cells expressing receptors or not (0 μg) was assessed using two-way ANOVA followed by Sidak’s post-tests (**** p < 0.0001; ns, not statistically significant).

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